L. Alphey

Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti.

This study shows that expressing cifA and cifB genes from Wolbachia strain wAlbB in Aedes aegypti testes induces CI-like sterility, which can be rescued by maternal cifA expression.

Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti

This paper improves genetic manipulation techniques in Aedes aegypti by introducing transgenic lines for Cre-mediated recombination and a plasmid-based PhiC31 integrase system for stable transgene integration.

A Zika virus-responsive sensor-effector system in Aedes aegypti

This paper describes a genetic system in Aedes aegypti mosquitoes that selectively eliminates ZIKV-infected individuals to reduce vectorial capacity.

Fake it to break it: mimicking superinfection exclusion disrupts alphavirus infection and transmission in the yellow fever mosquito Aedes aegypti

The paper demonstrates that mimicking superinfection exclusion in mosquitoes reduces alphavirus replication and transmission.

A multiplexed, confinable CRISPR/Cas9 gene drive propagates in caged Aedes aegypti populations

The paper tests a CRISPR/Cas9 gene drive in caged Aedes aegypti populations, showing it increases from 50% to 89% carrier frequency despite fitness costs.

CRISPR/Cas-9 mediated knock-in by homology dependent repair in the West Nile Virus vector Culex quinquefasciatus Say

This paper demonstrates the first successful germline gene knock-in in Culex quinquefasciatus using CRISPR/Cas9 and homology-dependent repair, achieving a 2.8% transformation rate with a recessive lethal phenotype.

Quantifying and modelling the acquisition and retention of lumpy skin disease virus by haematophagus insects reveals clinically but not subclinically-affected cattle are promoters of viral transmission and key targets for control of disease outbreaks.

This study quantifies LSDV acquisition and retention by vector insects and models transmission, finding clinical cattle are key promoters of viral spread and highlighting the need for targeted control strategies.