M. Caglayan

Structural and biochemical characterization of LIG1 during mutagenic nick sealing of oxidatively damaged ends at the final step of DNA repair

This study characterizes LIG1's structural and biochemical interactions with oxidatively damaged DNA during the final steps of base excision repair.

Uncovering nick DNA binding by LIG1 at the single-molecule level

This study investigates how LIG1 binds to nick DNA at the single-molecule level, revealing specific and non-specific binding dynamics and the role of the N-terminal region in nick search.

Structures of LIG1 active site mutants reveal the importance of DNA end rigidity for mismatch discrimination

This study investigates how mutations in LIG1's active site affect its ability to discriminate between correct and mismatched DNA ends, revealing the role of DNA end rigidity in ligation fidelity.

Structures of LIG1 uncover the mechanism of sugar discrimination against a ribonucleotide at 3'- and 5'-end of the nick DNA

This study determines the structures of LIG1/nick DNA complexes with 3- or 5-ribonucleotides to reveal how LIG1 discriminates against ribonucleotides at DNA ends.

Structures of LIG1 engaging with mutagenic mismatches inserted by polβ in base excision repair

This study determines the X-ray structures of LIG1 with mismatched DNA substrates to elucidate how LIG1 discriminates between mutagenic and correct mismatches during base excision repair.

DNA ligase I fidelity mediates the mutagenic ligation of pol β oxidized nucleotide insertion products and base excision repair intermediates with mismatches

This study shows that DNA ligase I fidelity affects the mutagenic ligation of pol β insertion products and repair intermediates with mismatches, and how APTX and FEN1 remove 5-AMP blocks.