Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry
T. Y. H. Bui, E. De Zitter, B. Moeyaert, L. Pecqueur, B. Y. Srinivasu, A. Economou, M. Fontecave, L. Van Meervelt, P. Dedecker, B. Pedre
The paper investigates oxygen-induced chromophore degradation in rsCherry, identifying cleavage of the p-hydroxyphenyl ring and formation of cyclic structures as the cause of fluorescence loss.
bioRxiv · Jan 13, 2023Biophysics