M. J. Tyska

Intestinal tuft cells assemble a cytoskeletal superstructure composed of co-aligned actin bundles and microtubules

This study uses advanced microscopy to reveal the ultrastructural organization of tuft cell cytoskeleton, showing co-aligned actin bundles and microtubules.

Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epithelia

This study shows that loss of CDHR2 disrupts intermicrovillar adhesion complexes, leading to impaired junctional complexes and barrier function in transporting epithelia.

Mitotic spindle positioning protein (MISP) is an actin bundler that senses ADP-actin and binds near the pointed ends of filaments

This study shows MISP binds preferentially to ADP-actin filaments near their pointed ends, forming parallel bundles and modulating microvilli structure.

Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions

This study shows that intermicrovillar adhesion complexes stabilize nascent microvilli at epithelial cell margins through transjunctional interactions, leading to their accumulation in medial regions.

Protrusion growth driven by myosin-generated force

This study shows that myosin-generated force can drive protrusion growth, independent of cargo delivery or barbed end elongation factors.

Mitotic Spindle Positioning (MISP) is an actin bundler that selectively stabilizes the rootlets of epithelial microvilli

The paper identifies MISP as an actin bundler that stabilizes microvillus rootlets and reveals its interaction with ezrin to maintain bundle structure.

Direct visualization of epithelial microvilli biogenesis

This study uses live imaging to visualize microvillus biogenesis in epithelial cells, identifying key temporal steps and factors involved in their formation and collapse.

A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions and function

The paper develops a heterologous in-cell assay to study intermicrovillar adhesion complex interactions and function, revealing a mechanism for length matching of surface protrusions.

SPECIES-DEPENDENT HETEROPHILIC AND HOMOPHILIC CADHERIN INTERACTIONS IN INTESTINAL INTERMICROVILLAR LINKS

The paper presents X-ray structures of PCDH24 and CDHR5 extracellular domains and identifies species-specific adhesive units for homophilic and heterophilic interactions.

Non-muscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli

This study identifies non-muscle myosin-2C as a key regulator of microvilli length by controlling actin turnover in the terminal web.

SETD2 is an actin lysine methyltransferase

This study identifies SETD2 as an actin lysine methyltransferase that regulates actin polymerization and cell migration through methylation of the actin cytoskeleton.

PACSIN2-dependent apical endocytosis regulates the morphology of epithelial microvilli

This study shows that PACSIN2 regulates microvilli morphology via apical endocytosis, with loss of PACSIN2 causing structural defects in intestinal brush borders.

Actin dynamics drive microvillar motility and clustering during brush border assembly

This study shows that microvilli move actively during brush border formation, driven by actin dynamics, leading to their clustering and organization.