F. Schneider

Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

Deep learning for histopathological subtyping and grading of lung adenocarcinoma

This study develops CNNs to subtype and grade lung adenocarcinoma, achieving high accuracy and outperforming most pathologists in grading prediction.

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes

The paper optimizes fluorescence lifetime imaging (FLIM) for mechanosensitive Flipper probes to measure membrane tension and long-range forces in cells, tissues, and organs.

The kinase occupancy of T-cell coreceptors reconsidered

The paper re-evaluates kinase occupancy of T-cell coreceptors CD4 and CD8β, finding CD4-Lck interaction is stoichiometric while CD8β-Lck is substantial, with structural differences influencing binding.

Single-cell resolution unravels spatial alterations in metabolism, transcriptome and epigenome of ageing liver

The paper investigates age-related spatial changes in metabolism, transcriptome, and epigenome in the murine liver using multi-omics approaches.

Single organoid RNA-sequencing reveals high organoid-to-organoid variability

This study uses RNA-seq to show that organoids vary significantly between individuals within a culture, but not between batches, suggesting microenvironmental effects drive this variability.

Targeting the translational machinery in gastrointestinal stromal tumors (GIST) - a new therapeutic vulnerability

This study shows that homoharringtonine (HHT) effectively inhibits KIT-dependent GIST growth by reducing protein synthesis, even in imatinib-resistant models.

Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5

The paper characterizes the diffusion and interaction dynamics of PEX5 in the cytosol using advanced microscopy techniques, revealing a novel interaction partner.

rasterdiv - an Information Theory tailored R package for measuring ecosystem heterogeneity from space: to the origin and back

The paper introduces an R package called rasterdiv for measuring ecosystem heterogeneity using remotely sensed data and demonstrates its application in revealing hidden heterogeneity patterns.

Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells

The paper investigates how membrane protein aggregation and immobilization affect lipid order in T cells, revealing lipid-mediated recruitment of signaling proteins like Lck.

Evaluation of the performance of SARS-CoV-2 serological tools and their positioning in COVID-19 diagnostic strategies.

This study evaluates the performance of SARS-CoV-2 serological tools across different panels and timepoints, finding varying sensitivities and specificities with poor agreement between assays.

Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

This study shows that anti-GFP nanobodies alter the fluorescence properties, mobility, and organization of GFP-tagged proteins by binding to non-emissive oligomers and promoting their disassembly into monomers.

High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy

This paper demonstrates that high photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy by using advanced detection equipment.

Nanoscale dynamics of cholesterol in the cell membrane

This study uses advanced fluorescence imaging to examine cholesterol dynamics in cell membranes, finding faster diffusion in live cells compared to model membranes.

Nanoscale spatio-temporal diffusion modes measured by simultaneous confocal and STED imaging

This study introduces LIESS-FCS, a method that simultaneously measures diffusion in confocal and STED modes to reveal nanoscale diffusion characteristics in live cell membranes.

Polarity sensitive probes for super resolution STED microscopy

This study evaluates polarity-sensitive dyes in super resolution STED microscopy to probe nanoscale membrane heterogeneity in cell-derived vesicles, live cells, and virus particles.