Single-cell transcriptomic assessment of cellular phenotype stability in human precision-cut lung slices
N. I. Winters, C. J. Taylor, C. S. Jetter, J. E. Camarata, A. J. Gutierrez, L. T. Bui, J. J. Gokey, M. Bacchetta, N. E. Banovich, J. M. S. Sucre, J. A. Kropski
The paper compares single-cell transcriptomic profiles of human PCLS after five days of culture to freshly isolated lung tissue, finding changes in endothelial and alveolar epithelial cell programs but stable immune cell profiles.
bioRxiv · Aug 19, 2021 · PreprintCell Biology