Author

P. W. Zandstra

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Immunology· 3 Bioengineering· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 6 Research Focus:cell type annotation· 5 Approach:experimental assay· 4 Assay:bulk RNA-seq· 3 Approach:statistical model· 3 Approach:deep learning· 2 Research Focus:differential expression· 2 Research Focus:gene regulatory network inference· 2

Frequent Co-Authors

J. M. Edgar· 4 shared M. C. Major· 3 shared C. Zimmerman· 2 shared E. L. Castle· 2 shared M. I. Ibanez-Rios· 2 shared Y. S. Michaels· 2 shared A. Forrow· 1 shared A. Hagner· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

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4 shared papersImmunology

J. M. Edgar

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Time- and lineage-resolved transcriptional profiling uncovers gene expression programs and clonal relationships that underlie human T lineage specification

Y. S. Michaels, M. C. Major, B. Bonham-Carter, J. Zhang, T. Heydari, J. M. Edgar, L. Greenstreet, R. Vilarrasa-Blasi, S. Kim, E. L. Castle, A. Forrow, M. I. Ibanez-Rios, C. Zimmerman, Y. Chung, T. Stach, , P. W. Zandstra

This study uses time-resolved single-cell RNA sequencing to uncover gene expression programs and clonal relationships during human T lineage specification.

bioRxiv · Oct 6, 2023 · PreprintImmunology

Sex biased human thymic architecture guides T cell development through spatially defined niches

L. N. Stankiewicz, F. M. Rossi, P. W. Zandstra, K. Salim, E. A. Flaschner, Y. X. Wang, J. M. Edgar, B. Z. Lin, G. C. Bingham, M. C. Major, R. D. Jones, H. Blau, E. Rideout, M. K. Levings

The paper maps sex differences in thymic niches using spatial multi-omics to reveal how sex influences T cell development and immune function.

bioRxiv · Apr 13, 2023Immunology

DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells

Y. S. Michaels, J. M. Edgar, M. C. Major, E. L. Castle, C. Zimmerman, T. Yin, A. Hagner, C. Lau, M. I. Ibanez-Rios, D. J. H. F. Knapp, P. W. Zandstra

This study identifies DLL4 and VCAM1 as critical factors enhancing T cell progenitor emergence from human pluripotent stem cells during the endothelial-to-hematopoietic transition.

bioRxiv · Nov 26, 2021Bioengineering

Inflammatory Cytokines Regulate T-cell Development from Blood Progenitor Cells in a Stage and Dose-Specific Manner

J. M. Edgar, P. W. Zandstra

This study identifies IL-3 and TNF as enhancers of proT-cell differentiation and expansion in vitro and develops a three-stage assay for generating mature T-cells.

bioRxiv · Jan 18, 2021 · PreprintImmunology

Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm

D. Dziedzicka, M. Tewary, A. Keller, L. Tilleman, L. Prochazka, J. Östblom, E. Couvreu De Deckersberg, C. Markouli, S. Franck, F. Van Nieuwerburgh, C. Spits, P. W. Zandstra, K. Sermon, M. Geens

The paper shows that endogenous suppression of WNT signaling in hPSCs reduces differentiation efficiency towards definitive endoderm, and that increasing WNT or inhibiting PI3K/AKT can overcome this block.

bioRxiv · Jul 3, 2020Cell Biology

A 96-well culture platform enables longitudinal analyses of engineered human skeletal muscle microtissue strength

M. E. Afshar, H. Y. Abraha, M. A. Bakooshli, S. Davoudi, N. Thavandiran, K. Tung, H. Ahn, H. Ginsberg, P. W. Zandstra, P. M. Gilbert

The paper introduces a 96-well platform for culturing human skeletal muscle microtissues to study their strength and response to drugs over time.

bioRxiv · Feb 28, 2019Bioengineering