Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes
Helena Rawsthorne, Fernando Calahorro, Patricia G. Izquierdo, Philippe Tardy, Thomas Boulin, Lindy M. Holden-Dye, Vincent M. O'Connor, James Dillon
The paper demonstrates that using co-CRISPR marker genes in C. elegans can lead to hidden genomic rearrangements that affect gene function, emphasizing the need for sequencing co-CRISPR genes after CRISPR editing.
PLoS ONE · Jan 18, 2022Peer-reviewedGenetics