R. R. Pompano

Mitigating reactive oxygen species production and increasing gel porosity improves lymphocyte motility and fibroblast spreading in photocrosslinked gelatin-thiol hydrogels

This study shows that reducing reactive oxygen species and increasing hydrogel porosity improves cell viability and function in photocrosslinked gelatin-thiol hydrogels.

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

This paper develops an optical method to measure spatially resolved oxygen consumption in ex vivo lymph node slices, revealing higher OCR in T cell regions compared to other areas.

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

This paper provides a tutorial on designing and fabricating bio-microfluidic devices using resin 3D printing, addressing challenges like resolution, mechanical stability, cytotoxicity, and optical compatibility.

Platinum chemotherapy induces lymphangiogenesis in cancerous and healthy tissues that can be prevented with adjuvant anti-VEGFR3 therapy

Detergent wash improves vaccinated lymph node handling ex vivo

This paper introduces a detergent wash method to improve the handling of reactive lymph nodes ex vivo, reducing tissue loss during slicing without affecting immune function.

Acute lymph node slices are a functional model system to study immunity ex vivo

This study validates live murine lymph node slices as a functional ex vivo model to study immune responses while preserving spatial organization and dynamic accessibility.

Generation of nonlinear and spatially-organized 3D cultures on a microfluidic chip using photoreactive thiol-ene and methacryloyl hydrogels

This paper develops a microfluidic method to create 3D cell cultures with precise spatial organization using photoreactive hydrogels and demonstrates its effectiveness with human T cells.

Spatially Resolved Measurement of Dynamic Glucose Uptake in Live Ex Vivo Tissues

This paper develops a method to measure dynamic glucose uptake in live tissue slices using fluorescent glucose analogues and microscopy, revealing spatial patterns in lymph nodes.