R. V. Pappu

This study uses theory and computation to show how ligands regulate scaffold phase behavior by binding to specific sites, affecting condensate stability and structure.

The paper introduces a computational approach to predict sequence-specific phase diagrams for intrinsically disordered proteins by combining simulations with Gaussian cluster theory.

The paper identifies 42 phosphorylation sites on NaV1.5 channels in mouse hearts and shows their roles in regulating channel expression and gating.

The paper identifies sequence features of mixed-charge domains that govern nuclear speckle condensation and function in mRNA processing.

LASSI simulates phase transitions of multivalent proteins using a lattice model, generating insights into condensate formation and design.

This study shows that spontaneous interactions between proteins and RNA can form multi-layered condensates with distinct material properties and specific amino acid and RNA sequence dependencies.

The paper measures NDC80 binding to microtubules in living cells and finds it is regulated by centromere tension and phosphorylation of Hec1.

The paper investigates the structure of Httex1 with expanded polyQ and finds that its tadpole-like conformation increases interactions in cells, potentially contributing to neurotoxicity.

This study shows that disordered linkers in multivalent proteins control the relationship between phase separation and gelation through their length and sequence features.