M. K. Rosen

Advanced Surface Passivation for High-Sensitivity Studies of Biomolecular Condensates

This paper introduces a surface passivation method using Pluronic F127 to reduce non-specific binding and enable high-precision analysis of biomolecular condensates.

Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct

This study identifies molecular features of BRD4-NUT and its interaction with p300 that drive condensate formation and gene expression, showing overlapping yet distinct roles of p300 domains.

Small Molecule Properties Define Partitioning into Biomolecular Condensates

The paper quantifies small molecule partitioning into biomolecular condensates and develops a machine learning model to predict partitioning based on physicochemical properties.

Quantitative reconstitution of yeast RNA processing bodies

This study reconstitutes yeast RNA processing bodies from purified components, showing that their composition and dynamics can be recapitulated quantitatively.

Coupling of protein condensates to ordered lipid domains determines functional membrane organization

This study shows that protein condensates and lipid domains couple to organize T-cell membrane function during activation.

Synergistic Phase Separation of Two Pathways Promotes Integrin Clustering and Integrin Adhesion Complex Formation

This study shows that p130Cas and FAK undergo phase separation, promoting integrin clustering and IAC formation through synergistic interactions.

In Diverse Conditions Intrinsic Chromatin Condensates Have Liquid-like Material Properties

This study shows that intrinsic chromatin condensates exhibit liquid-like properties under diverse conditions, challenging previous claims of their solidity.

Poly-glutamine-dependent self-association as a potential mechanism for regulation of androgen receptor activity

This study investigates how poly-glutamine sequences in the androgen receptor (AR) N-terminal domain influence its activity and interactions with KDM4A, suggesting a role for phase separation in regulating AR function in prostate cancer.

A chromatin phase transition protects mitotic chromosomes against microtubule perforation

This study shows that global histone deacetylation during mitosis induces a chromatin phase transition that protects chromosomes from microtubule perforation and ensures proper segregation.

The role of sigma-1 receptor in organization of endoplasmic reticulum signaling microdomains

This study investigates how the sigma-1 receptor (S1R) forms cholesterol-enriched microdomains in the ER and modulates signaling through changes in membrane thickness and cholesterol concentration.

Phase Separation Can Increase Enzyme Activity by Concentration and Molecular Organization

This study shows that phase separation can enhance enzyme activity through both concentration effects and molecular organization in synthetic condensates.

Inhibition of CRISPR-Cas12a DNA Targeting by Nucleosomes and Chromatin

This study shows that nucleosomes inhibit CRISPR-Cas12a binding and cleavage, with DNA unwrapping enhancing accessibility and cleavage rates.

Structure-function properties in disordered condensates

The paper studies client recruitment in biomolecular condensates using poly-SUMO and poly-SIM proteins, finding that filament structure influences functional client binding.

A quantitative inventory of yeast P body proteins reveals principles of compositional and specificity

The paper quantifies yeast P body protein concentrations, finding only 7 proteins are highly concentrated, while others are lower, and shows sequence elements drive Dcp2 localization.

The mechanisms of dynamin-actin interaction

This study identifies a noncanonical role for dynamin in cell-cell fusion by directly bundling actin filaments through GTPase-dependent interactions.

Phosphorylation of Nephrin induces phase separated domains that move through actomyosin contraction

This study shows phosphorylated Nephrin forms phase-separated domains at the plasma membrane, which move via actomyosin contraction.

Organization and Regulation of Chromatin by Liquid-Liquid Phase Separation

This study shows that chromatin undergoes liquid-liquid phase separation under physiological conditions, driven by histone tail interactions and modulated by acetylation and H1, leading to genome compaction and organization.

A Composition-Dependent Molecular Clutch Between T Cell Signaling Clusters and Actin

The paper investigates how Nck and WASP mediate the movement of LAT condensates across actin networks during T cell activation.

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins

This study shows that disordered linkers in multivalent proteins control the relationship between phase separation and gelation through their length and sequence features.

Intrinsically disordered regions contribute promiscuous interactions to RNP granule assembly

The paper shows that intrinsically disordered regions contribute promiscuous interactions to RNP granule assembly, working with specific interactions to form large cellular structures.