T. Mittag

Optical characterization of molecular interaction strength in protein condensates

The paper introduces optical techniques to measure molecular interaction strength and physical properties of protein condensates, showing how sequence variations and environmental factors affect these properties.

Tunable metastability of condensates reconciles their dual roles in amyloid fibril formation

The paper shows that separable interactions in A1-LCD allow tunable metastability of condensates, which can suppress amyloid fibril formation even with ALS mutations.

Design of intrinsically disordered protein variants with diverse structural properties

This paper introduces a physics-based algorithm to design intrinsically disordered proteins with specified structural properties, demonstrating high accuracy in predicting compaction and phase separation behavior.

Crowder titrations enable the quantification of driving forces for macromolecular phase separation

This paper demonstrates how crowder titrations can quantify intrinsic driving forces for macromolecular phase separation by measuring saturation concentrations and second virial coefficients.

Sequence-specific interactions determine viscoelasticity and aging dynamics of protein condensates

This study investigates how sequence-specific interactions influence the viscoelastic properties and aging dynamics of protein condensates formed by prion-like low-complexity domains.

Phase Separation in Mixtures of Prion-Like Low Complexity Domains is Driven by the Interplay of Homotypic and Heterotypic Interactions

This paper investigates phase separation of mixtures of prion-like low complexity domains using simulations and experiments, finding that homotypic and heterotypic interactions drive condensate formation.

Reduction of oligomer size modulates the competition between cluster formation and phase separation of the tumor suppressor SPOP

This study shows that reducing SPOP oligomer size shifts its function from cluster formation to phase separation, enhancing ubiquitination activity and providing insights into separation-of-function mutations.

Conformational and oligomeric states of SPOP from small-angle X-ray scattering and molecular dynamics simulations

This study characterizes SPOP's conformational and oligomeric states using SAXS and molecular dynamics simulations, revealing rigid helical structures with flexible linker regions.

Quantifying coexistence concentrations in multi-component phase-separating systems using analytical HPLC

This paper develops an analytical HPLC method to quantify coexisting phase concentrations in multi-component phase-separating systems, enabling reconstruction of coexistence curves.

Phase separation of Myc differentially regulates gene transcription

The paper investigates how phase separation of N-myc affects gene transcription and finds that only a small fraction of N-myc-regulated genes are influenced by this process.

Condensates of disordered proteins have small-world network structures and interfaces defined by expanded conformations

This study shows that disordered protein condensates form small-world networks with interface-specific conformational expansions and orientations.

Deciphering how naturally occurring sequence features impact the phase behaviors of disordered prion-like domains

The paper identifies how specific amino acid compositions influence phase separation in prion-like domains and develops a model to simulate these behaviors.

Microfluidic characterization of liquid-liquid phase separation

The paper introduces a microfluidic chip to measure phase separation of biomolecules with reduced sample quantity and improved statistical accuracy.

Interplay of folded domains and the disordered low-complexity domain in mediating hnRNPA1 phase separation

This study investigates how folded domains of hnRNPA1 modulate phase separation behavior of its disordered low-complexity domain, showing salt-dependent effects through electrostatic interactions and solubility changes.

Connecting coil-to-globule transitions to full phase diagrams for intrinsically disordered proteins

The paper introduces a computational approach to predict sequence-specific phase diagrams for intrinsically disordered proteins by combining simulations with Gaussian cluster theory.

Integrating multiple experimental data to determine conformational ensembles of an intrinsically disordered protein

The paper integrates NMR, SAXS, and smFRET data to determine the conformational ensembles of Sic1 and pSic1, showing consistency across methods and validating their structural models.

Protein network structure enables switching between liquid and gel states

The paper uses analytic theory to show how protein interactions determine phase separation behavior, revealing that gel formation is driven by entropy rather than binding energy.