R. Zarivach

Asgard ESCRT-III and VPS4 reveal evolutionary conserved chromatin binding properties of the ESCRT machinery

The paper shows that Asgard and human ESCRT-III/VPS4 proteins bind chromatin and form nuclear foci, indicating conserved chromatin-binding properties across evolution.

SAMase of bacteriophage T3 inactivates E. coli methionine S-adenosyltransferase by forming hetero-polymers

This paper shows that bacteriophage T3's SAMase degrades SAM and inhibits E. coli's MAT by forming hetero-polymers, leading to metabolic disruption.

Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated heterozygous deletion

This study uses CRISPR-Cas9 to delete one allele of LeishIF4E2 in Leishmania, revealing its role in morphology and proteome regulation, but not general translation.

The metal binding site composition of the cation diffusion facilitator protein MamM cytoplasmic domain impacts its metal responsivity

This study investigates how mutations in the metal binding sites of the MamM CTD affect its conformation and metal binding properties using X-ray crystallography and Trp-fluorescence spectrometry.

The cation diffusion facilitator protein MamM's cytoplasmic domain exhibits metal-type dependent binding modes and discriminates against Mn2+

This study investigates how the cytoplasmic domain of the CDF protein MamM binds different metals, showing it discriminates against Mn2+ and supports a role for CTDs in metal selectivity.

Integrative model to coordinate the oligomerization and aggregation mechanisms of CCL5

This study identifies two dimer-dimer interactions and one aggregation mechanism in CCL5 oligomerization using NMR and biophysical analyses.

The inter-dimeric interface controls function and stability of Ureaplasma urealiticum methionine S-adenosyltransferase

This study shows that the inter-dimeric interface of U. urealiticum MAT regulates catalytic activity and structural stability through conformational changes induced by product binding.

Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine

This study genetically encodes acetyl-lysine into STAT3 to investigate its effects on structure and DNA binding, revealing that acetylation does not directly alter these properties but may require additional factors for transcriptional regulation.

Transition metal binding selectivity in proteins and its correlation with the phylogenomic classification of the cation diffusion facilitator protein family

The paper analyzes DDMC binding propensities and correlates them with the phylogenetic classification of the CDF protein family to understand metal selectivity in transporters.