Rengifo-Gonzalez Monica

The paper shows pneumococcal infection activates innate immune genes in only 1-4% of lung epithelial cells, contrasting with E. coli's 40% response, and identifies prostaglandin signaling as protective during invasive disease.

This study uses CRISPRi-seq to identify genes affecting antibiotic sensitivity across diverse pneumococcal strains, revealing conserved and lineage-specific vulnerabilities.

This paper develops mbPE, a prime editing system for Streptococcus pneumoniae that enables precise genome editing without donor DNA, achieving high selection efficiencies for point mutations, deletions, and insertions.