Axel B. Janssen

A functional genetic landscape of antibiotic sensitivity across the pneumococcal pangenome reveals conserved and lineage-specific vulnerabilities

This study uses CRISPRi-seq to identify genes affecting antibiotic sensitivity across diverse pneumococcal strains, revealing conserved and lineage-specific vulnerabilities.

Strain-resolved CRISPRi-Seq reveals conserved antibiotic vulnerabilities in Staphylococcus aureus

The paper uses CRISPRi-Seq to identify conserved antibiotic vulnerabilities in S. aureus strains, revealing gene targets for antimicrobial therapies.

CRISPRi-seq in Haemophilus influenzae reveals genome-wide and medium-specific growth determinants

This paper introduces a CRISPRi-seq platform in Haemophilus influenzae to study gene fitness and essentiality, revealing medium-specific growth determinants and introducing a genome annotation resource.

Make-or-break prime editing for bacterial genome engineering

This paper develops mbPE, a prime editing system for Streptococcus pneumoniae that enables precise genome editing without donor DNA, achieving high selection efficiencies for point mutations, deletions, and insertions.

PneumoBrowse 2: An integrated visual platform for curated genome annotation and multiomics data analysis of Streptococcus pneumoniae

PneumoBrowse 2 provides an updated integrated platform for genome annotation and multi-omics data analysis of Streptococcus pneumoniae, enhancing research into its biology and potential treatments.

An efficient in vivo-inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studies

This paper develops a doxycycline-inducible CRISPRi system for GAS to enable efficient gene repression and presents a genome browser for GAS genetic features.