Vincent de Bakker

A TmaT-AftD interaction is required for the CmpL4 mycomembrane biogenesis pathway in Corynebacterium glutamicum.

This paper identifies the TmaT-AftD interaction as essential for mycolate transport via the CmpL4 pathway and AG synthesis in Corynebacterium glutamicum.

Staphylococcus aureus Mnh1 cation/proton antiporter promotes phagocytic uptake and intracellular survival in human monocytes

This study identifies Mnh1 as a key cation/proton antiporter promoting S. aureus phagocytic uptake and intracellular survival in human monocytes, suggesting it as a therapeutic target.

Epithelial innate immune sensing of pneumococci is inherently restricted to a small cellular minority across species and infection niches

The paper shows pneumococcal infection activates innate immune genes in only 1-4% of lung epithelial cells, contrasting with E. coli's 40% response, and identifies prostaglandin signaling as protective during invasive disease.

A potential role for acyl-phosphate in the coordination of phospholipid and lipopolysaccharide synthesis in Escherichia coli

The paper identifies PlsX as a suppressor of cell elongation defects in E. coli, suggesting acyl-phosphate regulates LPS synthesis by inhibiting LpxC.

A functional genetic landscape of antibiotic sensitivity across the pneumococcal pangenome reveals conserved and lineage-specific vulnerabilities

This study uses CRISPRi-seq to identify genes affecting antibiotic sensitivity across diverse pneumococcal strains, revealing conserved and lineage-specific vulnerabilities.

Multi-omics profiling reveals atypical sugar utilization and identifies a key membrane composition regulator in Streptococcus pneumoniae

This study uses multi-omics profiling to reveal atypical sugar utilization and identify a key membrane composition regulator in Streptococcus pneumoniae under infection-mimicking conditions.

In vivo genome-wide CRISPRi-Seq in Streptococcus pneumoniae reveals host-adaptive pathways essential for meningitis and identifies new therapeutic targets

This study uses in vivo CRISPRi-Seq in zebrafish to identify host-adaptive pathways essential for pneumococcal meningitis and discover new therapeutic targets.

Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle

This study uses dual CRISPRi-Seq to identify genetic interactions in Streptococcus pneumoniae, revealing key genes in the cell cycle and new interactions in essential processes.

CRISPRi-seq in Haemophilus influenzae reveals genome-wide and medium-specific growth determinants

This paper introduces a CRISPRi-seq platform in Haemophilus influenzae to study gene fitness and essentiality, revealing medium-specific growth determinants and introducing a genome annotation resource.

Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae

The paper identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae using CRISPRi-seq and antibiotic combination therapy.

Genome-wide CRISPRi-seq identified ferredoxin-NADP reductase FprB as a synergistic target for gallium therapy in Pseudomonas aeruginosa

The paper uses CRISPRi-seq to identify FprB as a synergistic target for gallium therapy in Pseudomonas aeruginosa, enhancing gallium's efficacy against infections.

Genome-wide analysis of fitness determinants of Staphylococcus aureus during growth in milk

This study identifies 282 milk-essential genes in Staphylococcus aureus using CRISPRi-seq, highlighting purine biosynthesis, folate cycle, and metal acquisition as critical for growth in milk.

Strain-level and phenotypic stability contrasts with plasmid and phage variability in water kefir communities

This study examines microbial community stability and variability in water kefir, finding species-level stability but plasmid and phage diversity across generations and households.

PneumoBrowse 2: An integrated visual platform for curated genome annotation and multiomics data analysis of Streptococcus pneumoniae

PneumoBrowse 2 provides an updated integrated platform for genome annotation and multi-omics data analysis of Streptococcus pneumoniae, enhancing research into its biology and potential treatments.