Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets
Joost J.A.P.M. Wijnakker, Gijs J. F. van Son, Daniel Krueger, Willine J. van de Wetering, Carmen López‐Iglesias, Robin Schreurs, Fenna L. H. van Rijt, Sangho Lim, Lin Lin, Peter J. Peters, Ralph R. Isberg, Claudia Y. Janda, Wim de Lau, Hans C. Clevers
The paper shows that Invasin-coated plates enable long-term 2D expansion of epithelial organoids from multiple species, offering an alternative to Matrigel/BME.
Proceedings of the National Academy of Sciences · Dec 30, 2024Peer-reviewedCell Biology