Ralph R. Isberg

The transcriptional response of Yersinia pseudotuberculosis to macrophage-released metabolites during growth within synthetic microcolonies

This study investigates how Yersinia pseudotuberculosis responds to macrophage-released metabolites during growth in synthetic microcolonies, identifying nitrosative stress and itaconate as key factors.

Host CD59 Potentiates the Type III Secretion System in Yersinia pseudotuberculosis

This study shows CD59 supports Yersinia pseudotuberculosis T3SS function by maintaining plasma membrane dynamics and lipid microdomain organization.

RNA Quality Control Enables Antibiotic Tolerance

This study shows that RNA quality control through rny mutations enables antibiotic tolerance in Streptococcus pneumoniae by balancing transcriptional resilience and degradation.

Resistance, heteroresistance and fitness costs drive colistin treatment failure during Acinetobacter baumannii murine pneumonia

Massively parallel combination screen reveals small molecule sensitization of antibiotic-resistant Gram-negative ESKAPE pathogens

This study uses high-throughput screening to identify small molecules that enhance antibiotic efficacy against multidrug-resistant Gram-negative bacteria.

Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets

The paper shows that Invasin-coated plates enable long-term 2D expansion of epithelial organoids from multiple species, offering an alternative to Matrigel/BME.

Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway

The paper identifies LegA7 as a cysteine protease in L. pneumophila that regulates MAPK pathway activation through conserved residues and ankyrin repeats.

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.

The paper presents a yeast-based method to identify informative missense mutations in bacterial effector proteins and validates it using three effectors from Legionella pneumophila.

Entropy of a bacterial stress response is a generalizable predictor for fitness and antibiotic sensitivity.

This paper introduces transcriptomic entropy as a generalizable predictor of bacterial fitness and antibiotic sensitivity across species and stress conditions using RNA-Seq and experimental evolution data.

The landscape of intrinsic and evolved fluoroquinolone resistance in Acinetobacter baumannii includes suppression of drug-induced prophage replication

This paper identifies mutations that alter fluoroquinolone sensitivity in Acinetobacter baumannii and shows that preferential Topo IV poisoning leads to prophage induction and SOS response activation.