Author

S. C. Kowalczykowski

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 3 Biophysics· 1 Microbiology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:chromatin & epigenetic regulation· 4 Approach:experimental assay· 4 Organism:bacteria (other)· 2 Assay:cryo-EM· 1 Assay:DNA methylation· 1 Organism:human· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

A.-L. Soulet· 1 shared C. C. Dombrowski· 1 shared C. J. Wong· 1 shared E. Le Cam· 1 shared E. Margeat· 1 shared J. Bell· 1 shared J. C. Bell· 1 shared J. L. Plank· 1 shared

Activity by Year

2022-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 13 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

1 shared paperMicrobiology

A.-L. Soulet

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition

T. L. Pavankumar, C. J. Wong, Y. K. Wong, M. Spies, S. C. Kowalczykowski

The paper investigates how RecBn loads RecA onto ssDNA via a χ-dependent mechanism, showing that RecBn can function independently of its flexible linker.

bioRxiv · May 14, 2023Biochemistry

A programmable and site-specific anchoring system for biophysical analysis of DNA:protein interactions on large topologically closed DNA molecules

N. S. Gilhooly, S. C. Kowalczykowski

The paper develops a method to create topologically closed, supercoiled DNA molecules for biophysical analysis of protein-DNA interactions without requiring additional manipulation.

bioRxiv · May 12, 2023 · PreprintBiophysics

BRCA2 chaperones RAD51 to single molecules of RPA-coated ssDNA

J. C. Bell, C. C. Dombrowski, J. L. Plank, R. B. Jensen, S. C. Kowalczykowski

This study uses single-molecule techniques to show BRCA2 chaperones RAD51 to RPA-coated ssDNA, overcoming kinetic barriers to promote homologous recombination.

bioRxiv · Dec 28, 2022 · PreprintBiochemistry

Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae.

M. Hertzog, T. N. Perry, P. Dupaigne, S. Serres, V. Morales, A.-L. Soulet, J. Bell, E. Margeat, S. C. Kowalczykowski, E. Le Cam, R. Fronzes, P. Polard

This study characterizes the ATP-dependent assembly mechanism and cryoEM structure of RecA nucleofilaments from Streptococcus pneumoniae, revealing differences in ssDNA binding and HR activity compared to Escherichia coli.

bioRxiv · Jul 28, 2022 · PreprintMicrobiology

Direct Visualization of Translesion DNA Synthesis Polymerase IV at the Replisome

T. M. Pham, N. Gilhooly, K. J. Marians, S. C. Kowalczykowski

This paper shows DNA polymerase IV associates with the replisome in bacteria under unstressed conditions, slowing replication and reducing mutation risk during stress.

bioRxiv · Apr 23, 2022Biochemistry