S. P. Ouellette

Distinct impacts of each anti-anti-sigma factor ortholog of the chlamydial Rsb partner switching mechanism on development in Chlamydia trachomatis

This paper investigates the role of two anti-anti-sigma factors, RsbV1 and RsbV2, in the partner switching mechanism of Chlamydia trachomatis, showing that RsbV2 overexpression blocks secondary differentiation, reducing infectious EB production.

FLI1 localization to the chlamydial inclusion involves multiple mechanisms

This study investigates FLI1 localization to the chlamydial inclusion and identifies multiple recruitment mechanisms independent of LRRF1.

Identification of the alternative sigma factor regulons of Chlamydia trachomatis using multiplexed CRISPR interference

This study identifies the regulons of two alternative sigma factors in Chlamydia trachomatis by using CRISPR interference and RNA sequencing, showing their essential roles in late-stage development.

Targeted repression of DNA topoisomerase I by CRISPRi reveals a critical function for it in the Chlamydia trachomatis developmental cycle

This study uses CRISPRi to knock down topA in Chlamydia trachomatis, showing its critical role in developmental cycle and growth.

In vivo and In vitro Characterization of the ClpC AAA+ ATPase of Chlamydia trachomatis

This study characterizes the ClpC AAA+ ATPase of Chlamydia trachomatis using in vitro and in vivo approaches, revealing its essential role in chlamydial growth and identifying it as a potential target for anti-chlamydial agents.

Localized Cardiolipin Synthesis is Required for the Assembly of MreB During the Polarized Cell Division of Chlamydia trachomatis

Regulation of an iron-dependent repressor by tryptophan availability attenuates transcription of the tryptophan salvage genes in Chlamydia trachomatis

The paper shows that YtgR represses trpBA expression in Chlamydia trachomatis by sensing both iron and tryptophan levels, using a rare triple-tryptophan motif to regulate transcription termination.

The ClpX and ClpP2 Orthologs of Chlamydia trachomatis Perform Discrete and Essential Functions in Organism Growth and Development

This study shows that ClpX and ClpP2 are essential for Chlamydia trachomatis growth and differentiation, with distinct roles in developmental cycle progression.

Chlamydia trachomatis Encodes a Dynamic, Ring-Forming Bactofilin Critical for Maintaining Cell Size and Shape

This study identifies BacACT, a bactofilin in Chlamydia trachomatis, which forms a dynamic ring structure critical for maintaining cell size and shape during replication.

Critical Role for the Unique N-Terminus of Chlamydial MreB in Directing Its Membrane Association and Interaction with Elements of the Divisome

This paper identifies the unique N-terminal region of Chlamydia MreB as critical for its membrane association and interaction with divisome components, supporting its role as a substitute for FtsZ in cell division.

Inhibition of tRNA Synthetases Induces Persistence in Chlamydia

This study shows that tRNA synthetase inhibitors induce chlamydial persistence independently of IFN-gamma by mimicking amino acid starvation.

Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells

The paper uses APEX2 proximity labeling to identify Inc-Inc and Inc-host interactions at the chlamydial inclusion membrane in C. trachomatis infected human cells, revealing LRRF1 as a host protein interacting with multiple Incs.

Initial Characterization of the Two ClpP Paralogs of Chlamydia trachomatis Suggests Unique Functionality for Each

This paper characterizes two ClpP paralogs in Chlamydia trachomatis, finding they have distinct expression, interaction, and proteolytic activity patterns.