Sheila Q. Xie

The paper experimentally measures CTCF-binding affinity across 276,765 human DNA sequences and develops a deep learning model to predict and interpret binding strength based on sequence features.

The paper investigates the role of Dux and MERVL in ZGA, totipotency, and cell toxicity using CRISPRa and a 2C-GFP reporter system, finding that MERVL activation contributes to expanded fate potential but not all totipotency features, while Dux-induced toxicity is independent of MERVL and involves Noxa.

The paper identifies a dual enhancer-silencer element at the Cdx2 locus that controls transient Cdx2 expression during posterior body formation in mice.

This study shows that nucleolar maturation and rRNA synthesis are required to repress Dux and exit the 2-cell stage in embryos.

The paper investigates how promoter activation states influence the 3D folding of the HoxB locus in mouse ES cells using simulations and single-cell imaging.