Author

Lessly P. Sepulveda-Rincon

Imperial College LondonORCID 0000-0001-8654-7362Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Assay:bulk RNA-seq· 1 Research Focus:clustering· 1 Assay:DNA methylation· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1 Organism:mouse· 1

Frequent Co-Authors

Alessandro VitrioloiD· 1 shared Alessia Valenti· 1 shared Benedetta MudaiD· 1 shared Bryony J. LeekeiD· 1 shared Camille DioniD· 1 shared Cristina CheroniiD· 1 shared Dirk DormanniD· 1 shared Filippo PrazzoliiD· 1 shared

Activity by Year

2025-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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1 shared paperDevelopmental Biology

Alessandro VitrioloORCID

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2 papers

Recent·Most cited

High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans

Sarah Stucchi, Lessly P. Sepulveda-Rincon, Camille Dion, Gaja Matassa, Alessia Valenti, Cristina Cheroni, Alessandro Vitriolo, Filippo Prazzoli, George Young, Marco Tullio Rigoli, Riccardo Nagni, Martina Ciprietti, Benedetta Muda, Zoe Heckhausen, Petra Hájková, , Harry G. Leitch

This paper establishes a defined feeder-free system to convert human PGC-like cells to pluripotent stem cells, tracking transcriptional and epigenomic transitions using multi-omic analysis.

Stem Cell Reports · Dec 19, 2025Peer-reviewedDevelopmental Biology

CRISPRa-mediated disentanglement of the Dux-MERVL axis in the 2C-like state, totipotency and cell death

Paul Chammas, Sheila Q. Xie, Lessly P. Sepulveda-Rincon, Bryony J. Leeke, Marian H. Dore, Dirk Dormann, Ryan T. Wagner, Ning Chang, Peter L. Jones, Michael T. McManus, Mohammad Mahdi Karimi, George R. Young, Michelle Percharde

The paper investigates the role of Dux and MERVL in ZGA, totipotency, and cell toxicity using CRISPRa and a 2C-GFP reporter system, finding that MERVL activation contributes to expanded fate potential but not all totipotency features, while Dux-induced toxicity is independent of MERVL and involves Noxa.

Science Advances · Dec 19, 2025Peer-reviewedDevelopmental Biology