A saturating mutagenesis CRISPR-Cas9 mediated functional genomic screen identifies cis- and trans-regulatory elements of Oct4 in embryonic stem cells
Matthew C. Canver, Pratibha Tripathi, Michael J. Bullen, Moshe Olshansky, Yogesh Kumar, Lee H. Wong, Stephen J. Turner, Samuel Lessard, Luca Pinello, Stuart H. Orkin, Partha Pratim Das
The paper identifies cis- and trans-regulatory elements of Oct4 in mESCs using ctSCAN-SMS, showing CREs are more active and conserved than TREs.
Journal of Biological Chemistry · Sep 29, 2020Peer-reviewedGenomics