Matthew C. Canver

ONE-seq identifies population-specific off-target effects of CRISPR gene editors using global human genome sequence diversity.

The paper identifies cis- and trans-regulatory elements of Oct4 in mESCs using ctSCAN-SMS, showing CREs are more active and conserved than TREs.

CRISPResso2 is a software tool for analyzing genome editing data, providing allele-specific quantification and scalable alignment to assess editing efficiency and safety.

CRISPR-SURF deconvolves CRISPR tiling screen data to identify functional regulatory elements across CRISPR-Cas, CRISPRi, and CRISPRa experiments.

CRISPRO maps guide RNA functional scores to genomic and structural coordinates, enabling structure-function relationship analysis and improved guide RNA efficacy prediction.

The paper presents a protocol for designing, executing, and analyzing CRISPR genome editing experiments using CRISPOR and CRISPResso.