CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
N. C. Dalvie, T. R. Lorgeree, Y. Yang, S. A. Rodriguez-Aponte, C. A. Whittaker, J. A. Hinckley, J. J. Clark, A. M. Del Rosario, K. R. Love, J. C. Love
The paper uses CRISPR-Cas9 to knock out endogenous secreted proteins in Komagataella phaffii, increasing recombinant protein production by 20x.
bioRxiv · Apr 21, 2024 · PreprintBioengineering