J. C. Love

Differentiation and Expansion of Tumor-Infiltrating T Cell Clonotypes Occurs in the Spleen Following Immune Checkpoint Blockade

This study identifies spleen-resident intermediate-exhausted T cells as key to ICB efficacy, showing their expansion and differentiation into tumor-infiltrating clonotypes.

Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to vaccination

The paper develops a method to recover full-length BCR sequences from single-cell RNA libraries and applies it to study convergent B cell responses to vaccination in rhesus macaques.

Early cellular and molecular signatures correlate with severity of West Nile Virus infection

The paper identifies cellular and molecular signatures associated with varying severities of West Nile Virus infection using multi-omics analysis of immune responses.

Eradication of tumors with pre-existing antigenic heterogeneity by vaccine-mediated co-engagement of CAR T and endogenous T-cells

This study shows vaccine boosting of CAR T-cells enhances tumor eradication by promoting antigen spreading and endogenous T-cell engagement, even in antigen-negative tumors.

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

The paper improves recombinant protein secretion quality and titer in Komagataella phaffii by modifying the N-terminus to enhance steric accessibility of the signal peptide.

Identification of highly cross-reactive mimotopes for a public T cell response in murine melanoma

The paper identifies a dominant tumor-associated antigen in murine melanoma and shows that engineered mimotopes can induce cross-reactive T cell responses.

WAT3R: Recovery of T-Cell Receptor Variable Regions From 3' Single-Cell RNA-Sequencing

WAT3R recovers T-cell receptor variable regions from 3' scRNA-seq data, enabling T-cell clonotype reconstruction and analysis of TCR sequences in single cells.

A modular protein subunit vaccine candidate produced in yeast confers protection against SARS-CoV-2 in non-human primates

This paper describes a protein subunit vaccine candidate produced in yeast that protects non-human primates against SARS-CoV-2, showing high neutralizing antibody titers and reduced viral loads after challenge.

Modular development enables rapid design of media for alternative hosts

The paper introduces a modular media design approach for rapid optimization of cell growth and protein production in Pichia pastoris using high-throughput fermentations and an open-source automation tool.

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

This paper describes an engineered Komagataella phaffii strain for methanol-free production of SARS-CoV-2 RBD, achieving a 5x increase in secreted titer and enabling scalable manufacturing for vaccine development.

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

The paper engineers subunit antigens to improve vaccine manufacturing by reducing variants and enabling single-process production of a trivalent rotavirus vaccine.

Leukocyte dynamics after intracerebral hemorrhage in a living patient reveal rapid adaptations to tissue milieu

The paper uses single-cell RNA-sequencing to study leukocyte dynamics in a living patient after intracerebral hemorrhage, revealing rapid immune responses and adaptations to the hematoma microenvironment.

Multimodal profiling of lung granulomas reveals cellular correlates of tuberculosis control

The paper identifies cellular and temporal factors associated with bacterial control in tuberculosis granulomas using multimodal data.

Highly Efficient, Massively-Parallel Single-Cell RNA-Seq Reveals Cellular States and Molecular Features of Human Skin Pathology

The paper introduces an improved scRNA-Seq protocol, Seq-Well S^3, which enhances transcript capture and gene detection efficiency, and applies it to study inflammatory skin diseases.