N. C. Dalvie

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

The paper improves recombinant protein secretion quality and titer in Komagataella phaffii by modifying the N-terminus to enhance steric accessibility of the signal peptide.

A modular protein subunit vaccine candidate produced in yeast confers protection against SARS-CoV-2 in non-human primates

This paper describes a protein subunit vaccine candidate produced in yeast that protects non-human primates against SARS-CoV-2, showing high neutralizing antibody titers and reduced viral loads after challenge.

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

This paper describes an engineered Komagataella phaffii strain for methanol-free production of SARS-CoV-2 RBD, achieving a 5x increase in secreted titer and enabling scalable manufacturing for vaccine development.

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

The paper engineers subunit antigens to improve vaccine manufacturing by reducing variants and enabling single-process production of a trivalent rotavirus vaccine.