Modulating CRISPR-Cas genome editing using guide-complementary DNA oligonucleotides
Thomas Swartjes, Peng Shang, Dennis T.M. van den Berg, Tim Künne, Niels Geijsen, Stan J. J. Brouns, John van der OOST, Raymond H.J. Staals, Richard A. Notebaart
This study shows DNA oligonucleotides can inhibit Cas9 activity in human cells and in vitro by targeting the guide RNA and requiring a PAM-loop for effective inhibition.
The CRISPR Journal · Jul 20, 2022Peer-reviewedMolecular Biology