Raymond H.J. Staals

Establishment of titration-based control of DNA replication in Escherichia coli

This study investigates how titration of an active DnaA variant controls DNA replication in E. coli under different growth conditions, revealing limits and design principles for synthetic replication systems.

Deletion of the moe A gene in Flavobacterium IR1 drives structural color shift from green to blue and alters polysaccharide metabolism

The paper shows that deleting the moeA gene in Flavobacterium IR1 changes structural color from green to blue and affects polysaccharide metabolism.

A CRISPR-associated Rossman-fold Ring Nuclease and Adenosine Deaminase Fusion Allosterically Converts ATP to ITP

This paper identifies a CRISPR-associated protein, Cad1, that converts ATP to ITP in a cOA-dependent manner through an allosteric mechanism.

The Escherichia coli replication initiator DnaA is titrated on the chromosome

This study investigates DnaA titration on the E. coli chromosome and shows it controls free DnaA levels in a growth rate-dependent manner, preventing re-initiation during slow growth.

Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

This study optimizes a genome editing system for Cupriavidus necator H16, achieving high editing efficiency using an improved SIBR-Cas system.

Towards sequential conjugation-assisted laboratory evolution (SCALE) of Cas nucleases

The paper demonstrates that plasmid conjugation can be used for in vivo laboratory evolution of Cas nucleases by enabling cleaving variants to spread through bacterial cultures.

Modulating CRISPR-Cas genome editing using guide-complementary DNA oligonucleotides

This study shows DNA oligonucleotides can inhibit Cas9 activity in human cells and in vitro by targeting the guide RNA and requiring a PAM-loop for effective inhibition.