Stan J. J. Brouns

Anti-phage defence systems are enriched in multidrug-resistant Pseudomonas aeruginosa

The paper analyzes the defensome of Pseudomonas aeruginosa across clinical datasets, finding that multidrug-resistant isolates have more defence systems while cystic fibrosis isolates have fewer, with implications for phage therapy.

Molecular basis for anti-jumbo phage immunity by AVAST Type 5

This study identifies Avs5 systems as a conserved bacterial defense against jumbo phages, revealing their mechanism of recognizing and disrupting phage infection.

KlebPhaCol: A community-driven resource for Klebsiella research identified a novel gut phage order associated with the human gut

The paper introduces KlebPhaCol, a resource containing 53 phages and 74 Klebsiella isolates, including a novel gut phage order, to advance understanding of Klebsiella-phage interactions.

Discovery of phage defense systems through component modularity networks

The paper identifies phage defense systems by analyzing modular gene clusters, validating three new systems through experimental testing.

DepoCatalog: Mapping the Diversity of 105 Recombinant Klebsiella Phage Depolymerases Across Sequence, Structure, and Substrate Specificity

The paper catalogs 105 recombinant Klebsiella phage depolymerases, classifying them by sequence, structure, and substrate specificity, revealing diverse enzyme functions and evolutionary patterns.

De novo peptide sequencing with InstaNovo: Accurate, database-free peptide identification for large scale proteomics experiments

InstaNovo is a transformer model for de novo peptide sequencing that outperforms existing methods, enabling accurate identification of peptides and discovery of novel sequences and organisms.

Reduced prevalence of phage defense systems in Pseudomonas aeruginosa strains from Cystic Fibrosis Patients

This study compares phage defense systems in Pseudomonas aeruginosa strains from cystic fibrosis and non-cystic fibrosis patients, finding fewer defenses in CF strains.

Modulating CRISPR-Cas genome editing using guide-complementary DNA oligonucleotides

This study shows DNA oligonucleotides can inhibit Cas9 activity in human cells and in vitro by targeting the guide RNA and requiring a PAM-loop for effective inhibition.

Comprehensive PAM prediction for CRISPR-Cas systems reveals evidence for spacer sharing, preferred strand targeting and conserved links with CRISPR repeats

The paper maps CRISPR spacers and identifies PAM sequences, revealing strand targeting preferences and shared CRISPR components across systems.

Prophages are associated with extensive CRISPR-Cas auto-immunity

The paper investigates STS in bacterial genomes, finding they are common and often linked to prophages, with mechanisms to evade auto-immunity.