High-throughput competitive binding assay for targeting RNA with small molecules: discovery of new PreQ1 riboswitch ligands
Sophie E.L. Wintermans, Jana S Hoffmann, Victor van Kuijk, Yelda Durmus, Mariska D. Tacoma, Indy Broekhuizen, Antonius P. A. Janssen, Hans van den Elst, Bjorn R. van Doodewaerd, Paul P. Geurink, Marta Artola, René C. L. Olsthoorn
This paper develops a competitive binding HTS assay to identify small molecule ligands for bacterial PreQ1 riboswitches, discovering 4 hits with activity against multiple riboswitches and one showing translation inhibition.
Nucleic Acids Research · Aug 27, 2025Peer-reviewedBiochemistry