The Holdup Multiplex, an assay for high-throughput measurement of protein-ligand affinity constants using a mass-spectrometry readout
François Delalande, Søren Østergaard, Gergő Gógl, A. Cousido-Siah, Alastair G. McEwen, Yushi Men, Farida Salimova, Aurélien Rohrbacher, Camille Kostmann, Yves Nominé, Renaud Vincentelli, Pascal Eberling, Christine Carapito, Gilles Travé, Élodie Monsellier
The paper introduces a high-throughput mass-spectrometry-based assay to measure protein-ligand affinity constants, demonstrating its application to 14-3-3 isoforms and phosphopeptides.
Journal of the American Chemical Society · Mar 25, 2025Peer-reviewedSystems Biology