Sascha M. Kuhn

This study quantifies lipid sorting during clathrin-mediated endocytosis and finds that lipid asymmetry in the plasma membrane explains observed partitioning, with minimal selectivity for cytoplasmic leaflet lipids compared to non-vesicular trafficking.

The paper introduces GEQO biosensors for absolute analyte quantification in single cells without requiring specialized equipment, showing they account for buffering and retain time trace information.

This study shows that disrupting plasma membrane lipid asymmetry in mammalian cells alters cellular energetics by shifting metabolism from anabolic to catabolic states.