Nils Birkholz

This study compares CRISPR-Cas systems for RNA knockdown in rat sensory neurons, finding hfCas13d provides robust gene silencing with minimal toxicity.

The paper identifies structured RNA motifs and Aca proteins that regulate anti-CRISPR gene expression in bacteriophages, revealing new regulatory mechanisms beyond DNA-binding HTH regulators.

This paper identifies viral single-guide RNAs (vsgRNAs) that subvert bacterial CRISPR-Cas9 immunity by sequestering Cas9 and redirecting its activity, and shows they can enable genome editing in mammalian cells.