Guangchuan Wang

Perturb-DBiT co-sequences spatial transcriptomes and gRNAs on one tissue section, supporting genome-wide in vivo CRISPR screens in FFPE and fresh-frozen tumors across three cancer models.

The paper introduces PASTAS, a method for in vivo CRISPR screening in mice, and demonstrates its use in identifying cancer driver variants in liver tumors.

This study uses an AAV-mediated CRISPR screen in mice to identify functional suppressors of glioblastoma, revealing novel and canonical tumor suppressors and their interactions.

The paper uses Cpf1 crRNA arrays to map genetic interactions in vivo, identifying novel drivers and co-drivers of cancer transformation and metastasis.