Ryan Dz-Wei Chow

Brain-wide Genome Editing via STEP-RNPs for Treatment of Angelman Syndrome

This paper develops a non-viral genome-editing method called STEP-RNPs that effectively treats Angelman syndrome in mice and human neurons, showing functional genetic rescue without off-target effects.

Th1 cells express Tfh effector molecules and associate with B cells at the T-B borderfollowing viral infection

The paper identifies Th1 cells interacting with B cells at the T-B border in viral infections, suggesting they contribute to antibody production outside classical germinal centers.

Real-world evaluation of deep learning algorithms to classify functional pathogenic germline variants

This study evaluates deep learning models for classifying pathogenic germline variants in hereditary breast cancer using UK Biobank data, finding limited clinical utility for variants of uncertain significance.

High-fidelity enhanced AsCas12a knock-in mice for efficient multiplexed gene editing, disease modeling and orthogonal immunogenetics

Cas12a/Cpf1 knock-in mice enable efficient multiplexed immune cell engineering

The paper demonstrates efficient multiplexed gene editing in immune cells using Cas12a/Cpf1 knock-in mice and associated viral vectors.

The aging transcriptome and cellular landscape of the human lung in relation to SARS-CoV-2

This study examines age-related changes in the human lung transcriptome and cellular composition, linking them to increased COVID-19 severity in older adults.

Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing

The paper introduces PASTAS, a method for in vivo CRISPR screening in mice, and demonstrates its use in identifying cancer driver variants in liver tumors.

Direct in vivo mapping of functional suppressors in glioblastoma genome

This study uses an AAV-mediated CRISPR screen in mice to identify functional suppressors of glioblastoma, revealing novel and canonical tumor suppressors and their interactions.

Mapping in vivo genetic interactomics through Cpf1 crRNA array screening

The paper uses Cpf1 crRNA arrays to map genetic interactions in vivo, identifying novel drivers and co-drivers of cancer transformation and metastasis.