Timothy T. Harden

The paper defines the kinetic roles of three transcription factors in the Drosophila embryo by analyzing their effects on transcriptional output through live imaging.

The paper quantifies how shadow enhancers and their duplications combine transcriptional output in Drosophila embryos, showing variation in their computational behavior across expression patterns.

The paper shows that Caudal counter-represses Hunchback to enable even-skipped stripe 2 expression in Drosophila embryos, challenging the traditional view of Hunchback as a direct activator.