Brianda Areli Hernández-Morán

The paper introduces universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids using Cas9-induced double strand breaks and homology-independent repair.

The paper shows that tag fusions can have tissue-specific effects on protein stability in transgenic mice, depending on the cell and tissue environment.

This study identifies a critical genomic region on the X chromosome linked to anophthalmia in mice but finds no clear causative mutation, suggesting regulatory changes may underlie the phenotype.