Andrew J. Wood

Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids

The paper introduces universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids using Cas9-induced double strand breaks and homology-independent repair.

Divergent effects of target protein stabilisation versus overproduction on PROTAC activity

This study compares how stabilizing versus overproducing target proteins affects PROTAC activity using β-catenin as a model.

Tissue-specific consequences of tag fusions on protein expression in transgenic mice

The paper shows that tag fusions can have tissue-specific effects on protein stability in transgenic mice, depending on the cell and tissue environment.

Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

This paper develops CRISPR-engineered mouse lines for rapid and specific degradation of endogenous proteins in vivo, revealing stage-specific requirements for condensin complexes in lymphocyte division.

Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence repair outcome

The paper shows heterochromatin slows CRISPR-Cas9 mutagenesis but does not affect repair outcomes, with mutagenesis inhibition depending on Cas9 exposure levels.