Author

James T. Neal

Broad InstituteORCID 0000-0002-3962-8570Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:human· 3 Assay:CRISPR screen· 2 Research Focus:variant effect prediction· 2 Research Focus:benchmarking· 1 Research Focus:differential expression· 1 Research Focus:gene regulatory network inference· 1 Assay:scRNA-seq· 1

Frequent Co-Authors

Celeste DiaziD· 2 shared Christian R. FagreiD· 2 shared Julia BaumaniD· 2 shared Alice H. BergeriD· 1 shared Andrew J. AguirreiD· 1 shared Andrew O. GiacomelliiD· 1 shared Annabel K. SangreeiD· 1 shared Anne Elizabeth CarpenteriD· 1 shared

Activity by Year

2020-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 26 mutual co-author links

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2 shared papersGenomics

Celeste DiazORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

A genome-wide atlas of human cell morphology

Meraj Ramezani, Erin Weisbart, Julia Bauman, Avtar K Singh, John Yong, Maria Lozada, Gregory P. Way, Sanam L. Kavari, Celeste Diaz, Eddy Leardini, Gunjan Jetley, Jenlu Pagnotta, Marzieh Haghighi, Thiago M. Batista, Joaquín Pérez‐Schindler, , James T. Neal

The paper presents a genome-wide perturbation atlas using CRISPR-Cas9 knockouts to link genes to cellular phenotypes, identifying TMEM251/LYSET as a Golgi-resident protein involved in lysosomal enzyme trafficking.

Nature Methods · Jan 27, 2025Peer-reviewedGenomics

Massively parallel assessment of human variants with base editor screens

Ruth E. Hanna, Mudra Hegde, Christian R. Fagre, Peter C. DeWeirdt, Annabel K. Sangree, Zsofia M Szegletes, Audrey L Griffith, Marissa N. Feeley, Kendall R Sanson, Yossef Baidi, Luke W. Koblan, David R. Liu, James T. Neal, John G. Doench

This paper uses CRISPR-Cas9 base editors in pooled screens to assess the functional impact of human variants, identifying loss-of-function mutations and drug sensitivity/resistance mutations.

Cell · Feb 1, 2021Peer-reviewedGenetics

Massively parallel phenotyping of variant impact in cancer with Perturb-seq reveals a shift in the spectrum of cell states induced by somatic mutations

Oana Ursu, James T. Neal, Emily K. Shea, Pratiksha I. Thakore, Livnat Jerby‐Arnon, Lan Nguyễn, Danielle Ashley Dionne, Celeste Diaz, Julia Bauman, Mariam Mounir Mosaad, Christian R. Fagre, Andrew O. Giacomelli, Seav Huong Ly, Orit Rozenblatt–Rosen, William C. Hahn, , Jesse S. Boehm

The paper uses Perturb-seq to assess the phenotypic impact of somatic variants in cancer, revealing a continuum of gain-of-function KRAS variants.

bioRxiv · Nov 16, 2020 · PreprintGenomics