Henry H. Lee

A scalable transposon mutagenesis system for non-model bacteria

This paper develops a scalable transposon mutagenesis system for non-model bacteria, identifying functional configurations and applying them to study gene function in Comamonas testosteroni.

A genetic platform for a biocementation bacterium

This paper develops a genetic toolkit for Sporosarcina pasteurii, enabling genome modification and uncovering genes involved in biocementation.

Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes

This paper develops an automated platform using active learning to discover electroporation protocols for non-model microbes, achieving higher transformation efficiency for industrial chassis.

Functional genomics in a microbe that degrades and metabolizes PET plastic

This study develops genetic tools for Piscinibacter sakaiensis to enable functional genomics and engineering for PET degradation.

A reference set of functional plasmids for Vibrio natriegens

The paper identifies functional plasmids for Vibrio natriegens using the POSSUM toolkit, showing compatibility of multiple replicons in wild-type and engineered strains.

Predicting microbial growth conditions from amino acid composition

This paper develops genome-based models to predict microbial growth conditions using amino acid composition, showing high accuracy for oxygen tolerance, temperature, salinity, and pH.

Design and construction towards a pan-microbial toolkit

The paper designs and constructs a pan-microbial genetic toolkit using high-throughput methods to identify functional genetic parts in non-model microbes.

A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria

The paper introduces a scalable framework to identify functional origins of replication in non-model bacteria using a combinatorial plasmid library and next-generation sequencing.

Towards Practical and Robust DNA-based Data Archiving Using "Yin-Yang Codec" System

This paper introduces a DNA-based data archiving system called Yin-Yang Codec, which achieves high recovery rates and physical information density in both in vitro and in vivo storage.

Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York

The paper detects and characterizes the SARS-CoV-2 lineage B.1.526 in New York, showing its rapid growth and potential impact on neutralization.

Enzymatic DNA synthesis for digital information storage

This paper develops an enzymatic DNA synthesis method for digital information storage, demonstrating error-tolerant retrieval of 144 bits and validating kilobyte-scale storage with 1 bit per nucleotide.

Establishing a cell-free Vibrio natriegens expression system

This paper develops a cell-free expression system using Vibrio natriegens, achieving high protein yield through optimized culturing and extract preparation.

Recombineering in Vibrio natriegens

This study demonstrates that {lambda}-Red homologs from the SXT mobile element in Vibrio natriegens enable highly efficient recombineering, enabling genomic modifications and advancing genetic engineering capabilities.