Nili Ostrov

A scalable transposon mutagenesis system for non-model bacteria

This paper develops a scalable transposon mutagenesis system for non-model bacteria, identifying functional configurations and applying them to study gene function in Comamonas testosteroni.

A genetic platform for a biocementation bacterium

This paper develops a genetic toolkit for Sporosarcina pasteurii, enabling genome modification and uncovering genes involved in biocementation.

Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes

This paper develops an automated platform using active learning to discover electroporation protocols for non-model microbes, achieving higher transformation efficiency for industrial chassis.

Functional genomics in a microbe that degrades and metabolizes PET plastic

This study develops genetic tools for Piscinibacter sakaiensis to enable functional genomics and engineering for PET degradation.

A reference set of functional plasmids for Vibrio natriegens

The paper identifies functional plasmids for Vibrio natriegens using the POSSUM toolkit, showing compatibility of multiple replicons in wild-type and engineered strains.

Synthetic genomes unveil the effects of synonymous recoding

This study uses synthetic genomes to investigate how synonymous codon recoding affects organismal fitness and develops a workflow to overcome lethal effects of codon swaps.

Predicting microbial growth conditions from amino acid composition

This paper develops genome-based models to predict microbial growth conditions using amino acid composition, showing high accuracy for oxygen tolerance, temperature, salinity, and pH.

Design and construction towards a pan-microbial toolkit

The paper designs and constructs a pan-microbial genetic toolkit using high-throughput methods to identify functional genetic parts in non-model microbes.

A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria

The paper introduces a scalable framework to identify functional origins of replication in non-model bacteria using a combinatorial plasmid library and next-generation sequencing.

Establishing a cell-free Vibrio natriegens expression system

This paper develops a cell-free expression system using Vibrio natriegens, achieving high protein yield through optimized culturing and extract preparation.

Recombineering in Vibrio natriegens

This study demonstrates that {lambda}-Red homologs from the SXT mobile element in Vibrio natriegens enable highly efficient recombineering, enabling genomic modifications and advancing genetic engineering capabilities.