Michele C. Darrow

This paper introduces Adaptive Polishing, a machine learning-driven method for improving the consistency and thinness of cryo-lamellae during focused ion beam milling.

The paper uses cryo-electron tomography to study the molecular organization of the HeLa secretory pathway, revealing small dimeric complexes dominate interactions within the ER and Golgi.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

This paper reviews existing biological segmentation datasets and recommends improvements to make them more reusable following FAIR principles.

Okapi-EM is a Napari plugin that processes and analyses cryogenic serial FIB/SEM images by aligning slices, evaluating image quality, and mitigating charging artefacts.

Ot2Rec is a semi-automatic, extensible workflow for cryo-ET image processing that enables flexible, automated tomographic reconstruction through plugins.