Thomas S. Glen

Adaptive Milling: imaging feedback-driven automated fabrication of consistently thin cryo-lamellae

This paper introduces Adaptive Polishing, a machine learning-driven method for improving the consistency and thinness of cryo-lamellae during focused ion beam milling.

Charting the molecular landscape of neuronal organisation within the hippocampus using cryo electron tomography

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

Reduction of SEM charging artefacts in native cryogenic biological samples.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

Okapi-EM - a napari plugin for processing and analysing cryogenic serial FIB/SEM images

Okapi-EM is a Napari plugin that processes and analyses cryogenic serial FIB/SEM images by aligning slices, evaluating image quality, and mitigating charging artefacts.