Jake L. R. Smith

This paper demonstrates that cryogenic femtosecond pulsed laser ablation can efficiently prepare vitreous specimens of mammalian brain tissue for cryo-ET, enabling routine in situ cell biology of multicellular samples.

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

Ot2Rec is a semi-automatic, extensible workflow for cryo-ET image processing that enables flexible, automated tomographic reconstruction through plugins.