Michael Grange

This paper demonstrates that cryogenic femtosecond pulsed laser ablation can efficiently prepare vitreous specimens of mammalian brain tissue for cryo-ET, enabling routine in situ cell biology of multicellular samples.

This paper introduces a multi-magnification cryo-ET technique that combines low- and high-resolution data to study cellular structures across scales.

This study shows that early HIV-1 maturation steps, specifically partial Gag cleavage, promote Env clustering and fusion competence by reorganizing the membrane-proximal lattice.

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

This paper demonstrates xenon plasma focused ion beam milling for preparing high-pressure frozen specimens, achieving a 4.0 Å E. coli ribosome structure with minimal structural damage beyond 45 nm.

Okapi-EM is a Napari plugin that processes and analyses cryogenic serial FIB/SEM images by aligning slices, evaluating image quality, and mitigating charging artefacts.

Ot2Rec is a semi-automatic, extensible workflow for cryo-ET image processing that enables flexible, automated tomographic reconstruction through plugins.

The paper introduces hardware and software improvements to enhance cryo-FIB milling for cryo-electron tomography.