Vallo Varik

The paper develops tools for transposon mutagenesis in Bacteroidales to identify essential genes and functional elements across three gut bacteria.

This study identifies nitroxoline's broad antibacterial activity against Gram-negative bacteria, its mode of action involving metal homeostasis and outer membrane disruption, and resistance mechanisms across multiple species.

The paper maps antibiotic cross-resistance and collateral sensitivity interactions using chemical genetic data and validates findings experimentally.