Barbara Fasulo

This study investigates the role of the femaleless gene in Anopheles gambiae gametogenesis and its impact on CRISPR-Cas9 gene drive efficacy, showing that disrupting fle causes female sterility and limits gene drive spread.

This paper proposes a self-limiting gene drive strategy called MDFS to suppress malaria mosquito populations by causing female sterility while allowing fertile males to spread the trait.

The paper engineers sex distortion in Ceratitis capitata using CRISPR/Cas9 and Cas12a to bias sex ratios towards males.

The paper establishes Drosophila models to study two CRISPR/Cas9 sex distortion mechanisms, X-shredding and X-meddling, and their functional differences.