Stephen D. Fried

This study defines the composition and architecture of the synaptonemal complex building block in C. elegans and shows how its subunits assemble into higher-order structures.

This study measures proteome-wide translation errors in yeast and mouse, finding that fidelity varies by organism and tissue, increases with age in post-mitotic cells, and is regulated by tRNA pool remodeling.

This paper develops a looped-translation system in bacteria to synthesize ultra-large fibrous proteins, achieving molecular weights exceeding titin and enabling secretion.

This study determines the cryo-EM structure of autoinhibited kinesin-1 and identifies mechanisms of autoinhibition and activation.

This study identifies that structured domains in PGL-3 drive phase separation through oligomerization and interaction with RGG motifs, independent of RNA.

This study identifies a topologically misfolded form of E. coli phosphoglycerate kinase that is more stable than its native state and resists denaturation.

The paper determines cryo-EM structures of Arabidopsis thaliana Augmin, revealing its coiled-coil assembly, antiparallel dimerization, and NEDD1 binding mechanisms.

This study shows random 25-mer peptides with a prebiotically plausible amino acid alphabet can self-assemble into thermostable beta-rich nanostructures.

This study uses RNA-seq and Ribo-seq in Haloferax volcanii to identify genes and small RNAs with altered translation during oxidative stress, revealing new regulatory mechanisms.

The paper identifies cognition-associated structural changes in aged rat hippocampal proteins using LiP-MS and finds these proteins are less refoldable than non-CASC proteins.

The paper shows PAR induces a conformational switch in FUS, enabling sustained condensation through N-terminal PrLD interactions after PAR degradation.

The paper uses LiP-MS to show that chaperone dependency during in vitro refolding does not correlate with chaperone dependency during in vivo primary protein biogenesis.

The paper identifies protein surface chemistry as a determinant of desiccation tolerance and shows that modifying surface amino acids can confer tolerance to otherwise sensitive proteins.

FLiPPR improves LiP-MS data analysis by formalizing data imputation, merging data, and correcting multiple hypotheses, enhancing statistical trends and metadata collection.

DomainMapper accurately annotates protein domain structures, including non-contiguous and insertional domains, validated against AlphaFold data.