Nediljko Budiša

The paper develops a psychrophilic orthogonal translation system for expanding the genetic code in E. coli, showing improved efficiency and versatility compared to mesophilic and thermophilic systems.

This study identifies design rules for multigene cassettes in human skin cells to avoid promoter interference and improve immunomodulatory function.

This paper engineers Escherichia coli as an antibiotic-free whole-cell biocatalyst with surface-displayed PETases for efficient plastic degradation.

This paper develops a genomically integrated orthogonal translation system in E. coli, enabling non-canonical amino acid incorporation and creating a biosafe synthetic chassis for enhanced protein production.

This study evolves Escherichia coli to use fluorinated indoles as nutrients, converting them into fluorotryptophan and incorporating them into the proteome.

This paper uses adaptive laboratory evolution to replace tryptophan in E. coli with a synthetic analog, revealing that stress response inhibition, not translation rigidity, limits genetic code expansion.

This study shows that Escherichia coli can adapt to use fluorinated indoles as essential precursors for protein synthesis through few genetic mutations and significant regulatory and cellular changes.